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SAMPLES (6)
mace:id
Technology # Array version
# SEVERAL # # SEVERAL
Affymetrix # HGU 133 Plus 2
Affymetrix # MGU 74 Av2
Affymetrix # MoGene V1.0st
Affymetrix # Mouse 430A
Affymetrix # Rhesus
Agilent # AGHUMAN
Agilent # AGMOUSE
Applied Biosystems # HGS V1
Applied Biosystems # HGS V2
Applied Biosystems # MGS V1
Applied Biosystems # MGS V2
Applied Biosystems # RGS V1
Genopole SXB # SXBH1
Genopole SXB # SXBH2
Genopole SXB # SXBH3
Genopole SXB # SXBM1
Genopole SXB # SXBM2
Genopole SXB # SXBM3
Illumina # HumanHT-12 V4.0
Illumina # HUMANWG6v3
Illumina # MouseWG-6 v2.0
Species
# SEVERAL
Cercocebus atys
Chlorocebus sabaeus
Homo sapiens
Macaca mulatta
Macaca Nemestrina
Mus musculus
Pan troglodytes
Rattus norvegicus
Organ
# OTHER
# SEVERAL
Adenoid
Adrenal gland
Bladder
Blood
Blood vessel
Brain
Bronchi
Cervix
Embryo
Esophagus
Gallblader
Heart
Hypotalamus
Intestine
Kidney
Larynx
Liver
Lung
Lymph node
Mammary gland
Mussle
Pancreas
Parathyroid
Penis
Pharynx
Pineal gland
Pituitary gland
Prostate
Salivary gland
Seminal vesicle
Skin
Spinal cord
Spleen
Stomach
Test
Thymus
Thyroid
Tonsil
Trachea
Ureter
Uterus
Vagina
Vas deferens
Tissue
# OTHER
# SEVERAL
Bone Marrow
Connective - Dense Irregular Tissue (Collagen)
Connective - Dense Regular Tissue (Collagen)
Connective - Dense Regular Tissue (Elastic)
Connective - Loose Tissue (Adipose)
Connective - Loose Tissue (Areolar)
Connective - Loose Tissue (Reticular)
Epithelium - Simple (Columnar)
Epithelium - Simple (Cuboidal)
Epithelium - Simple (Pseudostratified)
Epithelium - Simple (Squamous)
Epithelium - Stratified (Columnar / Cuboidal)
Epithelium - Stratified (Squamous: Keratinized)
Epithelium - Stratified (Squamous: NonKeratinized)
Fluid - Blood
Fluid - Lymph
Gland - Endocrine Glands
Gland - Exocrine Glands (Ducts and Tubules)
Muscle - Non-striated
Muscle - Striated (Cardiac)
Muscle - Striated (Skeletal)
Nervous - Nerves
Nervous - Neurons (Bipolar)
Nervous - Neurons (Multipolar)
Nervous - Neurons (Unipolar)
Nervous - Receptors
Placenta
Stem cells
Supportive - Cartilage (Elastic)
Supportive - Cartilage (Fibrocartilage)
Supportive - Cartilage (Hyaline)
Supportive - Osseous (Compact)
Supportive - Osseous (Spongey)
Physiopathology
# HEALTHY
# OTHER
# SEVERAL
apoptosis
autocrine signaling
differentiation
drug response
electric response
endocrine signaling
environemental response
homeostasis
immune response
mechanic response
necrosis
paracrine signaling
proliferation
Type
# OTHER
# SEVERAL
conditional knockout
drug stress
electric stress
environmental stress
ground state
immune stress
knockdown RNAi
knockout
mechanic stress
stable transfection
time course
transient transfection
Name
Attached file
download project data file ('.map')
Attached file (see:
ruid website
)
download project data file ('.map' RUID converted)
Attached file
download raw data files ('.zip')
Attached file
download annotation files ('.zip')
User name
Arndt Benecke
Email
arndt@ihes.fr
Phone / Fax number
+33160926665 / +33160926609
Location
Institut des Hautes Etudes Scientifiques (Integrative Biology) - 35, route de Chartres - 91440 Bures sur Yvette, France
Scientific description
The complete sequencing of the human genome and its subsequent analysis revealed a predominant role for alternative splicing in the generation of proteome diversity. Splice switching oligonucleotides (SSOs) are a powerful and specific tool to experimentally control alternative splicing of endogenous messenger RNAs in living cells. SSOs also have therapeutic potential to treat diseases that are caused by aberrant splicing. The assignment of biological roles to alternative splicing events of currently unknown function promises to provide a largely untapped source of potential new therapeutic targets. Here we have developed a protocol that combines high sensitivity microarrays with the transfection of SSOs to monitor global changes in gene expression downstream of alternate, endogenous splice events. When applied to a well-characterized splicing event in the Bcl-x gene, the application of high sensitivity microarrays revealed a link between the induction of the Bcl-xS isoform and the repression of genes involved in protein synthesis. The strategy introduced herein provides a useful approach to define the biological impact of any given alternative splicing event on global gene expression patterns. Furthermore, our data provide the first link between Bcl-xS expression and the repression of ribosomal protein gene expression.
Technical description
Characteristics Hela cells + Control antisense RNA oligo Extracted molecule total RNA Extraction protocol RNA was extracted from the cell lines using RNeasy Midi Kit (Quiagen) according to the instructions provided by the manufacturer Label DIG-UTP Label protocol Digoxigenin-UTP labeled cRNA was generated and amplified from 2 μg of total RNA from each sample using Applied Biosystems NanoAmp RT-IVT Labeling Kit (P/N 4365715) according to the manufacturer’s protocol(P/N 4365710). Hybridization protocol Array hybridization was performed for 16 hrs at 55 oC. Pre-Hybridization, Hybridization, antibody binding and Chemiluminescence reaction were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) following the manufacturer’s protocol (P/N 4339627). Scan protocol image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629). Description The Bcl-xS transcription program. Data processing Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software v 1.1 (P/N 4336391). Median normalization and Probabilty for a gene to be expressed(A) were calculated with proprietary software.
References
Wilhelm E, Pellay FX, Benecke A, Bell B (2008) Determining the impact of alternative splicing events on transcriptome dynamics. BMC Res. Notes. 1:94.
Pubmed : http://www.ncbi.nlm.nih.gov/pubmed/18950505
previously published on GEO with acc. no.: GSE9851