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SAMPLES (12)

mace:id
Technology # Array version
Species
Organ
Tissue
Physiopathology
Type
Name

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User name	Jean-Claude Sirard
Email	Jean-Claude.Sirard@inserm.fr
Phone / Fax number	+33320877319 / +33320877888
Location	INSERM - Institut Pasteur de Lille (Center for Infection and Immunity of Lille, Team 8, INSERM U1019) - 1 rue du Pr. Calmette - 59019 Lille, France

Scientific description

Adaptive immunity depends on activation of antigen-presenting cells (APC) like dendritic cells. In lymphoid tissues, Toll-like receptors (TLR) that detect microbial signals are instrumental in the direct APC stimulation. In peripheral tissues including mucosa, TLR stimulation of structural cells is required to initiate immunity by transmitting signals to APCs. We previously showed that upon nasal administration of the TLR5 agonist flagellin, lung epithelial cells are the driving force essential to promote the adjuvant effect to co-administered antigens. We performed whole lung analysis after nasal flagellin administration to correlate gene expression to cell infiltration into the respiratory tract and to epithelial signals required for APC recruitment and activation. To this aim, lung were sampled at 0h (reference condition), 2h, 4h and 18h after nasal immunization.

Technical description

Total RNA was analyzed on the ABI Mouse Whole Genome Arrays (Applied Biosystems) as previously described (Eilebrecht et al. 2008). One single round of linear amplification was performed from 1Âµg of total RNA. cDNA synthesis, in vitro transcription, amplification, fragmentation, hybridization, staining, and scanning were performed as directed by the suppliers. Raw data, after having removed the signals stemming from the different control spots as well as failed measurements, were once more median normalized in log2-space.

References

Eilebrecht S*, Pellay FX*, OdenwÃ¤lder P, Brysbaert G, Benecke BJ, Benecke A (2008) EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection. BMC Res. Notes. 1:100.

References

Pubmed : http://www.ncbi.nlm.nih.gov/pubmed/18641341

Pubmed : http://www.ncbi.nlm.nih.gov/pubmed/ 20566828

Pubmed : http://www.ncbi.nlm.nih.gov/pubmed/19424969