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SAMPLES (54)

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Technology # Array version
Species
Organ
Tissue
Physiopathology
Type
Name

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User name	Arndt Benecke
Email	arndt@ihes.fr
Phone / Fax number	+33160926665 / +33160926609
Location	Institut des Hautes Etudes Scientifiques (Integrative Biology) - 35, route de Chartres - 91440 Bures sur Yvette, France

Scientific description

TAF6d, a splicing variant of the general transcription factor TAF6, is able to induce apoptosis when expressed in cancer cell lines. To induce cell death, TAF6d elicits a gene expression program involving signaling pathways implicated in apoptosis and oncogenesis, including the oxidative stress response, p53, Notch, integrins, angiogenesis or apoptosis pathways. The work presented in this manuscript allowed the elucidation of the molecular mechanisms underlying TAF6d induced apoptosis. Thus, a time course analysis in HeLa WS cells lead to the identification of BIM and NOXA as putative effectors in this cell death. Functional assays showed the implication of this pathway of the apoptosis and confirmed the role of BIM and NOXA. In addition, our results suggest that this model is transposable to other cancer cell lines (Saos-2, MDA-MB-231, Hs-578T and Panc-1).

Technical description

Hela ws cell line was maintained in culture in Dulbecco's modified Eagle's medium
supplemented with 2.5% fetal calf serum and 2.5% calf serum.
RNA extraction (RNeasy Qiagen) for microarray analysis as well as quality control were performed as previously described [2,22]. Applied
Biosystems HGS V2 arrays [23] were hybridized, washed, and exposed according to the
protocols by the technology provider. Raw data were quality controlled [24], and normalized using NeONORM (k-parameter=0.2) [25]. Longitudinal analysis was performed using a Kohonen-maps based classifier as described in [26], using the CDS statistical test [27].

Reference

Delannoy A, Wilhelm E, Eilebrecht S, Alvarado Cuevas EM, Benecke AG, Bell B (2017) The TAF6delta gene expression program at temporal resolution. submitted.