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SAMPLES (54)
mace:id
Technology # Array version
# SEVERAL # # SEVERAL
Affymetrix # HGU 133 Plus 2
Affymetrix # MGU 74 Av2
Affymetrix # MoGene V1.0st
Affymetrix # Mouse 430A
Affymetrix # Rhesus
Agilent # AGHUMAN
Agilent # AGMOUSE
Applied Biosystems # HGS V1
Applied Biosystems # HGS V2
Applied Biosystems # MGS V1
Applied Biosystems # MGS V2
Applied Biosystems # RGS V1
Genopole SXB # SXBH1
Genopole SXB # SXBH2
Genopole SXB # SXBH3
Genopole SXB # SXBM1
Genopole SXB # SXBM2
Genopole SXB # SXBM3
Illumina # HumanHT-12 V4.0
Illumina # HUMANWG6v3
Illumina # MouseWG-6 v2.0
Species
# SEVERAL
Cercocebus atys
Chlorocebus sabaeus
Homo sapiens
Macaca mulatta
Macaca Nemestrina
Mus musculus
Pan troglodytes
Rattus norvegicus
Organ
# OTHER
# SEVERAL
Adenoid
Adrenal gland
Bladder
Blood
Blood vessel
Brain
Bronchi
Cervix
Embryo
Esophagus
Gallblader
Heart
Hypotalamus
Intestine
Kidney
Larynx
Liver
Lung
Lymph node
Mammary gland
Mussle
Pancreas
Parathyroid
Penis
Pharynx
Pineal gland
Pituitary gland
Prostate
Salivary gland
Seminal vesicle
Skin
Spinal cord
Spleen
Stomach
Test
Thymus
Thyroid
Tonsil
Trachea
Ureter
Uterus
Vagina
Vas deferens
Tissue
# OTHER
# SEVERAL
Bone Marrow
Connective - Dense Irregular Tissue (Collagen)
Connective - Dense Regular Tissue (Collagen)
Connective - Dense Regular Tissue (Elastic)
Connective - Loose Tissue (Adipose)
Connective - Loose Tissue (Areolar)
Connective - Loose Tissue (Reticular)
Epithelium - Simple (Columnar)
Epithelium - Simple (Cuboidal)
Epithelium - Simple (Pseudostratified)
Epithelium - Simple (Squamous)
Epithelium - Stratified (Columnar / Cuboidal)
Epithelium - Stratified (Squamous: Keratinized)
Epithelium - Stratified (Squamous: NonKeratinized)
Fluid - Blood
Fluid - Lymph
Gland - Endocrine Glands
Gland - Exocrine Glands (Ducts and Tubules)
Muscle - Non-striated
Muscle - Striated (Cardiac)
Muscle - Striated (Skeletal)
Nervous - Nerves
Nervous - Neurons (Bipolar)
Nervous - Neurons (Multipolar)
Nervous - Neurons (Unipolar)
Nervous - Receptors
Placenta
Stem cells
Supportive - Cartilage (Elastic)
Supportive - Cartilage (Fibrocartilage)
Supportive - Cartilage (Hyaline)
Supportive - Osseous (Compact)
Supportive - Osseous (Spongey)
Physiopathology
# HEALTHY
# OTHER
# SEVERAL
apoptosis
autocrine signaling
differentiation
drug response
electric response
endocrine signaling
environemental response
homeostasis
immune response
mechanic response
necrosis
paracrine signaling
proliferation
Type
# OTHER
# SEVERAL
conditional knockout
drug stress
electric stress
environmental stress
ground state
immune stress
knockdown RNAi
knockout
mechanic stress
stable transfection
time course
transient transfection
Name
Attached file
download project data file ('.map')
Attached file (see:
ruid website
)
download project data file ('.map' RUID converted)
Attached file
download raw data files ('.zip')
Attached file
download annotation files ('.zip')
User name
Nicolas Tchitchek
Email
nicolas.tchitchek@ihes.fr
Phone / Fax number
0033610887604 /
Location
Institut des Hautes Études Scientifiques (Systems Epigenomics Group) - 35, route de Chartres - 91440 Bures-sur-Yvette, FRANCE
Scientific description
From abstract: The 1918 pandemic influenza virus was the most devastating infectious agent in human history, causing fatal pneumonia and an estimated 20-50 million deaths worldwide. Previous studies indicate a prominent role of the HA gene in efficient replication and high virulence of the 1918 virus in mice. It is, however, still unclear whether the high replication ability or the 1918 HA gene is required for 1918 virus to exhibit high virulence in mice. Here, we examined the biological properties of reassortant viruses between the 1918 virus and a contemporary human H1N1 virus (A/Kawasaki/173/2001; K173) in a mouse model. In addition to the 1918 HA, we demonstrated the role of viral RNA replication complex in efficient replication of viruses in mouse lungs, whereas only HA gene is responsible for lethality in mice. Global gene expression profiling of infected mouse lungs revealed 1918 HA was sufficient to induce transcriptional changes similar to the 1918 virus, despite difference in lymphocyte gene expression. Increased expression of genes associated with the acute phase response and protein ubiquitination pathway were enriched during 1918 and 1918HA/K173 infections, whereas reassortant viruses bearing the 1918 viral RNA polymerase complex induced transcriptional changes similar to K173 virus. Taken together, these data suggest HA and the viral RNA polymerase complex are critical determinants of Spanish influenza pathogenesis, but only HA, and not the viral RNA polymerase complex and NP, is responsible for extreme host responses observed in mice infected with the 1918 influenza virus.
Technical description
From material & method section: Mice were intranasally inoculated with 106 PFU of virus (n = 45) or inoculated with phosphate-buffered saline (n = 6), and whole lungs were collected on days 1, 3, and 5 p.i. from infected animals (n = 3/time point/group) and from control animals (n = 2/time point) for microarray analysis. Two of 3 mice infected with either 1918 virus or 1918HA/K173 virus died by day 5 p.i. RNA was isolated from total lung homogenates and fluorescently labeled probes were generated from each sample using Agilent one-color LowInput Quick Amp Labeling Kit (Agilent Technologies). Individual cRNA samples were hybridized to oligonucleotide microarrays for gene expression profiling using Whole Mouse Genome Microarray Kit (G4122A; Agilent Technologies), as previously described. RNA isolated from control animal lungs served as an uninfected reference. Quantitative reverse transcription-PCR was performed on mouse lung samples using a primer-probe set targeting the viral M sequence. A single biological replicate infected with 1918PB1/K173 virus at day 3 p.i. was confirmed to be uninfected and the sample was discarded from the dataset prior to analysis.
Reference
Watanabe T, Tisoncik-Go J, Tchitchek N, Watanabe S, Benecke AG, Katze MG, Kawaoka Y. 1918 HA and the viral RNA polymerase complex enhance viral pathogenicity, but only HA induces aberrant host responses in mice. J Virol. (2013)
Pubmed : http://www.ncbi.nlm.nih.gov/pubmed/23449804