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SAMPLES (112)
mace:id
Technology # Array version
# SEVERAL # # SEVERAL
Affymetrix # HGU 133 Plus 2
Affymetrix # MGU 74 Av2
Affymetrix # MoGene V1.0st
Affymetrix # Mouse 430A
Affymetrix # Rhesus
Agilent # AGHUMAN
Agilent # AGMOUSE
Applied Biosystems # HGS V1
Applied Biosystems # HGS V2
Applied Biosystems # MGS V1
Applied Biosystems # MGS V2
Applied Biosystems # RGS V1
Genopole SXB # SXBH1
Genopole SXB # SXBH2
Genopole SXB # SXBH3
Genopole SXB # SXBM1
Genopole SXB # SXBM2
Genopole SXB # SXBM3
Illumina # HumanHT-12 V4.0
Illumina # HUMANWG6v3
Illumina # MouseWG-6 v2.0
Species
# SEVERAL
Cercocebus atys
Chlorocebus sabaeus
Homo sapiens
Macaca mulatta
Macaca Nemestrina
Mus musculus
Pan troglodytes
Rattus norvegicus
Organ
# OTHER
# SEVERAL
Adenoid
Adrenal gland
Bladder
Blood
Blood vessel
Brain
Bronchi
Cervix
Embryo
Esophagus
Gallblader
Heart
Hypotalamus
Intestine
Kidney
Larynx
Liver
Lung
Lymph node
Mammary gland
Mussle
Pancreas
Parathyroid
Penis
Pharynx
Pineal gland
Pituitary gland
Prostate
Salivary gland
Seminal vesicle
Skin
Spinal cord
Spleen
Stomach
Test
Thymus
Thyroid
Tonsil
Trachea
Ureter
Uterus
Vagina
Vas deferens
Tissue
# OTHER
# SEVERAL
Bone Marrow
Connective - Dense Irregular Tissue (Collagen)
Connective - Dense Regular Tissue (Collagen)
Connective - Dense Regular Tissue (Elastic)
Connective - Loose Tissue (Adipose)
Connective - Loose Tissue (Areolar)
Connective - Loose Tissue (Reticular)
Epithelium - Simple (Columnar)
Epithelium - Simple (Cuboidal)
Epithelium - Simple (Pseudostratified)
Epithelium - Simple (Squamous)
Epithelium - Stratified (Columnar / Cuboidal)
Epithelium - Stratified (Squamous: Keratinized)
Epithelium - Stratified (Squamous: NonKeratinized)
Fluid - Blood
Fluid - Lymph
Gland - Endocrine Glands
Gland - Exocrine Glands (Ducts and Tubules)
Muscle - Non-striated
Muscle - Striated (Cardiac)
Muscle - Striated (Skeletal)
Nervous - Nerves
Nervous - Neurons (Bipolar)
Nervous - Neurons (Multipolar)
Nervous - Neurons (Unipolar)
Nervous - Receptors
Placenta
Stem cells
Supportive - Cartilage (Elastic)
Supportive - Cartilage (Fibrocartilage)
Supportive - Cartilage (Hyaline)
Supportive - Osseous (Compact)
Supportive - Osseous (Spongey)
Physiopathology
# HEALTHY
# OTHER
# SEVERAL
apoptosis
autocrine signaling
differentiation
drug response
electric response
endocrine signaling
environemental response
homeostasis
immune response
mechanic response
necrosis
paracrine signaling
proliferation
Type
# OTHER
# SEVERAL
conditional knockout
drug stress
electric stress
environmental stress
ground state
immune stress
knockdown RNAi
knockout
mechanic stress
stable transfection
time course
transient transfection
Name
Attached file
download project data file ('.map')
Attached file (see:
ruid website
)
download project data file ('.map' RUID converted)
Attached file
download raw data files ('.zip')
Attached file
download annotation files ('.zip')
User name
Béatrice Jacquelin
Email
beatrice.jacquelin@pasteur.fr
Phone / Fax number
0145688792 /
Location
Institut Pasteur (Unité de Régulations des Infections Rétrovirales) - 25-28, rue du Docteur Roux - Paris, France
Scientific description
SIVagm-infected African green monkeys (AGM) do not develop chronic T cell activation and AIDS, despite similar viral loads as HIV-1 infected humans and SIVmac-infected rhesus macaques (RM). To understand the underlying molecular basis, we longitudinally assessed the gene expression profiles of blood- and lymph node (LN)-derived CD4+ cells from AGMs and RMs in response to SIVagm and SIVmac infection, respectively. Gene expression was analyzed using human long oligonucleotide arrays which return a robust signal with CD4+ cell RNA from AGM (>13,500 genes) and RM (>14,500 genes). The molecular signature of acute infection was characterized by strong up-regulation of type I Interferon-Stimulated Genes (ISG). ISG expression returned to basal level after day 28 post-infection in AGMs but was sustained in RMs, especially in LN. ISG are a surrogate marker for IFN-alpha activity. Our results suggest that IFN-alpha levels and/or the associated responses are rapidly controlled in AGM. This control might be involved in the weak inflammatory response that characterizes primary SIVagm infection.
Technical description
In this serie, there are six AGM female (Chlorocebus sabaeus from Barbados), 3 years old, weighing 2.8–3.4 kg. AGM were intravenously infected with 250 TCID50 of SIVagm_sab92018 on day 0. 10ml of whole blood was collected from those animals under anaesthesia in heparinized tubes at 4 time points before infection (days -90, -70, -40 and -8) to get a robust baseline, 4 time points during the acute phase of the infection (days 1, 6, 14, 28) and 3 time points during the chronic phase (days 41, 65 and 115). PBMC were isolated freshly from blood samples by Ficoll procedures. Biopsies of peripheral Lymph nodes were performed by excision at 2 time points before infection (days -90, -8) and 6 time points post-infection for AGMs (days 1, 6, 14, 28, 65, 590). After careful removal of adhering connective and fat tissues, the LN fragments were mechanically disrupted on a sterile stainless steel mesh and filtered to collect LN mononuclear cells (LNMC). The recovered PBMCs and LNMCs were then washed, counted, and the the CD4+ cell subset was further isolated from those tissues using the MACS magnetic labeling system (Miltenyi Biotec, Germany) according to instructions of the manufacturer. Isolated cells consists of >95% of CD4+ T lymphocytes (CD3+ CD4+ cells). The total RNA was extracted and analyzed on the ABI Human Whole Genome Arrays v2.0. With the ABI array protocol 600ng of total RNA were used to generate sufficient hybridization signal.
References
Jacquelin B, Mayau V, Targat B, Liovat AS, Kunkel D, Petitjean G, Dillies MA, Roques P, Butor C, Silvestri G, Giavedoni LD, Lebon P, Barré-Sinoussi F, Benecke A, Muller-Trutwin M (2009) Strong but Rapidly Controlled Interferon Type I Response in Nonpathogenic SIV Infection of African Green Monkeys. J. Clinical Invest. doi:10.1172/JCI40093. in the press. -- see also published comment: Manches O, Bhardwaj N (2009) Resolution of immune activation defines nonpathogenic SIV infection. J. Clinical Invest. doi:10.1172/JCI41509.
Pubmed : http://www.ncbi.nlm.nih.gov/pubmed/19959873
Jacquelin B, Mayau V, Targat B, Liovat AS, Kunkel D, Petitjean G, Dillies MA, Roques P, Butor C, Giavedoni LD, Lebon P, Barré-Sinoussi F, Benecke A, Muller-Trutwin M (2009) Inflammatory control in AIDS-resistant non human primates. Retrovirology. 6:S2:123.